Originally from: lina
                        
FMD-3ABC bo-ov
Product description

The CHEKIT-FMD-3ABC bo-ov enzyme immunoassay (EIA) kit
provides a rapid, simple, sensitive and specific
method for detecting antibodies against the pathogen
responsible for Foot-and-Mouth-Disease (FMDV) in serum
or plasma samples of bovine and ovine origin.
This test allows to discriminate between samples from
infected (3ABC positive) and vaccinated (3ABC
negative) animals.

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Outline of the procedure

Microtiter plates are supplied pre-coated with
recombinant FMDV 3ABC viral antigen. Dilutions of the
samples to be tested are incubated in the wells of
these plates. Any antibody specific for 3ABC binds to
the antigen in the wells and forms an antigen/antibody
complex on the plate well surface. Unbound material is
removed from the wells by washing. A peroxidase
labelled anti-IgG-peroxidase- conjugate is added,
which binds to the antibodies of the sample complexed
with the 3ABC antigen.
Unbound conjugate is removed by washing, and the
chromogen-containing substrate is added to the wells.
The degree of colour which develops (optical density
measured at 405 nm, reference wavelength 492 nm) is
directly proportional to the amount of antibody
specific for 3ABC present in the sample. The
diagnostic relevance of the result is obtained by
comparing the optical density (OD) which develops in
wells containing the samples with the OD from the
wells containing the positive control.

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Kit content

kit of 5
microtiter plates "Bulk"
quantities
5 pcs. of 5 pcs. CHEKIT-FMD-3ABC microtiter plate,
coated with recombinant FMDV 3ABC protein.
1 x 1.0 ml of 1.0 ml CHEKIT-Anti-IgG-PO-Conjugate,
monoclonal,
labelled with horseradish peroxidase.
1 x 0.4 ml of 0.4 ml
CHEKIT-FMD-3ABC-bo-ov-Control-Serum, positive,
supplemented with 0.1% sodium azide as a preservative.

1 x 0.4 ml of 0.4 ml
CHEKIT-FMD-3ABC-bo-ov-Control-Serum, negative,
supplemented with 0.1% sodium azide as a preservative.

3 x 100 ml of 100 ml CHEKIT-FMD-3ABC-Sample-Diluent,
ready to use.
2 x 100 ml of 500 ml CHEKIT-10x-Concentrate, not
ready to use.
1 x 100 ml of 500 ml CHEKIT-Chromogen solution, ready
to use.
1 x 60 ml of 500 ml CHEKIT-Stopping solution, ready to
use.

http://www.bommeli.com/news/news_f_m.htm

http://www.bommeli.com/news/CHEKIT%20Detailed%20Product%20line%20_E_%205–8–2003.pdf